Test

In-vitro

  • Inflammation
  • Whitening
  • Wrinkles
  • Moisturizing
  • Antioxidation
  • Regeneration
  • Skin Barrier
  • Temperature Sensitization
  • Hair
  • Itching
  • Keratin Improvement
  • Phototoxicity
  • Cell Aging
Test Item Test Name Provision of Results
Inflammation NO assay Numeric
qPCR (IL-1β, COX-2, IL-6, TNF-α, iNOS)
COX-2 enzyme activity test
Western blotting (SPT)
Western blot
p-c-Jun / c-Jun
c-fos
p-NF-κB / NF-κB
Whitening Tyrosinase Inhibition assay Numeric
Melanin assay
Wrinkles qPCR (MITF, Tyrosinase, TRP-1, TRP-2) Numeric
Western blot (MITF)
Elastase Inhibition assay
qPCR (MMP-1)
Moisturizing qPCR (AQP-3, HAS-2) Numeric
Western blotting (AQP-3)
Antioxidation DPPH assay Numeric
FRAP assay
ABTS assay
Intracellular GSH contents
Western blot (NRF-2)
Regeneration Proliferation assay Numeric
Migration assay
Skin Barrier qPCR (Claudin-1, e-cadherin, Filaggrin, Involucrin, Loricrin, Caspase-14) Numeric
Temperature Sensitization Temperature sensitization related factors (TRPM8-Cooling) Numeric
Hair qPCR (KGF, VEGF, HGF, MYC, FGF-2) Numeric
Angiogenesis confirmation (Tube formation assay)
Itching qPCR (MDC, TARC, RANTES, CXCL10) Numeric
Keratin Improvement Protein degradation confirmation (Protease activity) Numeric
Phototoxicity In vitro 3T3 NRU Numeric
Cell Aging Cell aging enzyme staining (SA-β-galactosidase staining) Numeric
Aging cell phenotype (DAPI)
SASP factor setting